Manchester United defender Aaron Wan Pisaka has been knocked out of the England squad before facing Bulgaria and Kosovo in the Euro 2020 qualifier with a back injury.
"Aaron Wan Pisaka has left the England squad ... Manchester United right back has returned to his club with a back problem," the FA said in a statement. Of the 24 players, he does not plan to sign a replacement.
Wan Besaka was one of four players - Tyrone Minges, James Madison and Mason Mount - who have never played any internationals and joined Gareth Southgate's squad.
Bisaka was part of the England squad for this summer's U-21 Championship.
England will play Bulgaria in Wembley on September 7 and then face Kosovo three days later.