FC Barcelona said in a statement that the operation will take place in Turku, Finland under the supervision of the club's medical services.
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According to Catalan media, Sergi Roberto could be out for around four months.
FC Barcelona have indicated that they will issue a new medical statement after the operation.
Initially treated for a stretched quadriceps from which he suffered at the end of October, Sergi Roberto had resumed training with the rest of the group.
He would have been the victim of a relapse according to the Spanish media.
The Spaniard, who is one of Barca's four co-captains, has made 12 appearances this season.
He had already missed 37 matches last season due to several injuries, and was sidelined two weeks before the start of the season this summer with a rib injury.